© 2019 by Phenocell SAS - Photo credit : Kim Garell, F. Simonnet, Amelie Peschanski and the Phenocell Team

 

State-of-the-art bioassays,

taylor-made solutions & finely tuned projects for your R&D programs

All

bioassays

 
SEBOCYTE Bioassays
Using PCi-SEB, not immortalized, not genetically modified 

Cell proliferation/Survival

Ref. SEB-LCC

Endpoint: Nb live/dead cells

Method: Calcin AM - Annexin V*

Biological question: Cell proliferation - Survival

Androgen receptor activation 

Ref. SEB-ARS

Endpoint: Cell ratio intensity of staining

Method: BODIPY fluorescence

Biological question: Androgen receptor stimulation, sebogenesis

mRNA expression 

Ref. SEB-RNA

Endpoint: Relative expression level

Method: RT-qPCR

Biological question: Sebogenesis, acne

Cellular oxidative stress

Ref. SEB-CRX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question:  Oxidative stress response at cell level

Linoleic acid induction

Ref. SEB-LAS

Endpoint: Cell ratio intensity of staining

Method: BODIPY fluorescence

Biological question: PPAR stimulation, sebogenesis (response to linoleic acid)

Inflammation (secretion)

Ref. SEB-IL8

Endpoint: IL-8 concentration in cell supernatant

Method: ELISA

Biological question: PPAR stimulation, inflammation (arachidonic acid induction)

Mitochondrial Oxidative stress

Ref. SEB-MSX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question: Oxidative stress response at mitochondria level

Arachidonic acid induction

Ref. SEB-AAS

Endpoint: Cell ratio intensity of staining

Method: BODIPY fluorescence

Biological question: PPAR stimulation, sebogenesis (response to arachidonic acid)

Inflammation (Expression)

Ref. SEB-ICE

Endpoint: Expression of IL-8, IL-6 and TNF-alpha mRNA

Method: RT-qPCR

Biological question: PPAR stimulation, inflammation (arachidonic acid)

Apoptosis

Ref. SEB-TUN

Endpoint: Cell ratio intensity of staining

Method: TUNEL Fluorescent staining

Biological question: Apoptosis

Lipid content analysis

Ref. SEB-LCA

Endpoint: Lipid composition, quantification

Method: GC/MS

Biological question: Sebogenesis

 
MELANOCYTE Bioassays
Using our unique PCi-MEL with controlled phototype
(Asian, Caucasian, African)

Cell proliferation/Survival

Ref. MEL-LCC

Endpoint: Nb live/dead cells

Method: Calcin AM - Annexin V*

Biological question: Cell proliferation - Survival

Melanin content

Ref. MEL-MLN

Endpoint: Concentration in melanin

Method: OD475 reading - photometry

Biological question: Pigmentation

PMEL17 expression

Ref. MEL-PMEL17

Endpoint: PMEL17 immunolabeling

Method: Immuno-fluorescence

Biological question: Pigmentation

Cellular oxidative stress

Ref. MEL-CRX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question:  Oxidative stress response at cell level

Tyrosinase expression

Ref. MEL-TYR

Endpoint: Cell ratio intensity of staining

Method: Fluorescence imaging

Biological question: Pigmentation

TYRP1 expression

Ref. MEL-TYRP1

Endpoint: TYRP1 immunolabeling

Method: Immuno-fluorescence

Biological question: Pigmentation

Mitochondrial Oxidative stress

Ref. SEB-MSX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question: Oxidative stress response at mitochondria level

MITF expression

Ref. MEL-MITF

Endpoint: MITF immunolabeling

Method: Immuno-fluorescence

Biological question: Key transcription factor, melanogenesis

mRNA expression

Ref. MEL-RNA

Endpoint: Relative expression level

Method: RT-qPCR

Biological question: Pigmentation disorder, melanogenesis pathway

Apoptosis

Ref. MEL-TUN

Endpoint: Cell ratio intensity of staining

Method: TUNEL Fluorescent staining

Biological question: Apoptosis

PAX3 expression

Ref. MEL-PAX3

Endpoint: PAX3 immunolabeling

Method: Immuno-fluorescence

Biological question: Key transcription factor

 
RETINA PIGMENT EPITHELIUM CELL
Bioassays
PCi-RPE in unlimited quantities for reproducible assays

Cell proliferation/Survival

Ref. RPE-LCC

Endpoint: Nb live/dead cells

Method: Calcin AM - Annexin V*

Biological question: Cell proliferation - Survival

Melanin content

Ref. RPE-MEL

Endpoint: Concentration in melanin

Method: OD475 reading photometry

Biological question: Pigmentation

Outer retinal barrier

Ref. RPE-ORB

Endpoint: Resistance in Ohm/cm2

Method: Epithelial Volt/Ohm meter

Biological question: Epithelial barrier, retinal disorders

TYRP1 expression

Ref. RPE-TYRP1

Endpoint: TYRP1 immunolabeling

Method: Immuno-fluorescence

Biological question: Pigmentation

Cellular oxidative stress

Ref. RPE-CRX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question:  Oxidative stress response at cell level

Phagocytosis assay

Ref. RPE-PHAG

Endpoint: Cell ratio intensity of staining

Method: Fluorescence imaging

Biological question: Phagocytosis, retinal disorders

MITF expression

Ref. RPE-MITF

Endpoint: MITF immunolabeling

Method: Immuno-fluorescence

Biological question: Key transcription factor

mRNA expression 

Ref. RPE-RNA

Endpoint: Relative expression level. All genes

Method: RT-qPCR

Biological question: Retinal disorders

Mitochondrial Oxidative stress

Ref. RPE-MSX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question: Oxidative stress response at mitochondria level

VEGF secretion

Ref. RPE-VEGF

Endpoint: VEGF concentration

Method: OD reading by photometry

Biological question: Growth factor secretion, retinal disorders

ZO-1 expression

Ref. RPE-ZO1

Endpoint: ZO-1 immunolabeling

Method: Immuno-fluorescence

Biological question: Tight-junction, epithelial barrier

Apoptosis

Ref. RPE-TUN

Endpoint: Cell ratio intensity of staining

Method: TUNEL Fluorescent staining

Biological question: Apoptosis

PEDF secretion

Ref. RPE-PEDF

Endpoint: PEDF concentration

Method: OD reading by photometry

Biological question: Growth factor secretion, retinal disorders

PMEL17 expression

Ref. RPE-PMEL17

Endpoint: PMEL17 immunolabeling

Method: Immuno-fluorescence

Biological question: Pigmentation

Tailor-made projects
Looking for donor-specific iPSC ?
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Need unprecedented iPSC-derived cellular types ?
Need large amounts of cells, e.g. over 50 Mn ?
Looking for innovation ?
 

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