© 2019 by Phenocell SAS - Photo credit : Kim Garell, F. Simonnet, Amelie Peschanski and the Phenocell Team

 

Pharmacology

Cell stress

and survival

Cell stress facts

  • Human cells respond to adverse environmental conditions by a number of adaptive mechanisms that include anti-oxydant, .

  • Adaptation allows cell survival if the stress is not too intense or prolonged.

  •   Mitochondria plays an instrumental role in cell survival against oxydant stress.

Cellular stress-related assays

Cell proliferation/Survival

Ref. SEB-LCC

Endpoint: Nb live/dead cells

Method: Calcin AM - Annexin V*

Biological question: Cell proliferation - Survival

Cellular oxidative stress

Ref. MEL-CRX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question:  Oxidative stress response at cell level

Cell proliferation/Survival

Ref. MEL-LCC

Endpoint: Nb live/dead cells

Method: Calcin AM - Annexin V*

Biological question: Cell proliferation - Survival

Cellular oxidative stress

Ref. SEB-CRX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question:  Oxidative stress response at cell level

 

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