© 2019 by Phenocell SAS - Photo credit : Kim Garell, F. Simonnet, Amelie Peschanski and the Phenocell Team

Dermo-cosmetique

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RPE-based assays

 

Cell proliferation/Survival

Ref. RPE-LCC

Endpoint: Nb live/dead cells

Method: Calcin AM - Annexin V*

Biological question: Cell proliferation - Survival

Melanin content

Ref. RPE-MEL

Endpoint: Concentration in melanin

Method: OD475 reading photometry

Biological question: Pigmentation

Outer retinal barrier

Ref. RPE-ORB

Endpoint: Resistance in Ohm/cm2

Method: Epithelial Volt/Ohm meter

Biological question: Epithelial barrier, retinal disorders

TYRP1 expression

Ref. RPE-TYRP1

Endpoint: TYRP1 immunolabeling

Method: Immuno-fluorescence

Biological question: Pigmentation

Cellular oxidative stress

Ref. RPE-CRX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question:  Oxidative stress response at cell level

Phagocytosis assay

Ref. RPE-PHAG

Endpoint: Cell ratio intensity of staining

Method: Fluorescence imaging

Biological question: Phagocytosis, retinal disorders

MITF expression

Ref. RPE-MITF

Endpoint: MITF immunolabeling

Method: Immuno-fluorescence

Biological question: Key transcription factor

mRNA expression 

Ref. RPE-RNA

Endpoint: Relative expression level. All genes

Method: RT-qPCR

Biological question: Retinal disorders

Mitochondrial Oxidative stress

Ref. RPE-MSX

Endpoint: Cell ratio intensity of staining.

Method: Fluorescent staining

Biological question: Oxidative stress response at mitochondria level

VEGF secretion

Ref. RPE-VEGF

Endpoint: VEGF concentration

Method: OD reading by photometry

Biological question: Growth factor secretion, retinal disorders

ZO-1 expression

Ref. RPE-ZO1

Endpoint: ZO-1 immunolabeling

Method: Immuno-fluorescence

Biological question: Tight-junction, epithelial barrier

Apoptosis

Ref. RPE-TUN

Endpoint: Cell ratio intensity of staining

Method: TUNEL Fluorescent staining

Biological question: Apoptosis

PEDF secretion

Ref. RPE-PEDF

Endpoint: PEDF concentration

Method: OD reading by photometry

Biological question: Growth factor secretion, retinal disorders

PMEL17 expression

Ref. RPE-PMEL17

Endpoint: PMEL17 immunolabeling

Method: Immuno-fluorescence

Biological question: Pigmentation

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