© 2020 by Phenocell SAS - Photo credit : K. Garell, F. Simonnet, A. Peschanski and the Phenocell Team

Dermo-cosmetics

Pigmentation

Take advantage of standard phenotypic assays with Caucasian, African or Asian PCi-MEL

or order a tailor-made project

Pigmentation Facts

  • Hyperpigmentation is a skin condition that occur in people usually due to an unhealthy or higher production of a pigment called melanin, produced by melanocytes.

  • Hypopigmentation refers to a loss of melanin production related to genetic condition (albinism, vitiligo, atopic dermatitis, ...) or injury (burns).

  •   Voluntary modification of skin pigmentation can be cultural.

 

Pigmentation-related assays

Melanin determination

Ref. MEL-MLN

Endpoint: Melanin concentration

Method: OD475 reading - photometry

Biological question: Pigmentation

mRNA expression 

Ref. MEL-RNA

Endpoint: Relative expression level. All genes

Method: RT-qPCR

Biological question: Expression of markers in melanogenesis pathways

Tyrosinase expression

Ref. MEL-TYR

Endpoint: Cell ratio intensity of staining

Method: Fluorescence imaging

Biological question: Expression of key transcription factor, melanogenesis

MITF expression

Ref. MEL-MITF

Endpoint: MITF immunolabeling

Method: Fluorescence imaging

Biological question: Expression of key transcription factor,

melanogenesis